A simple sensitive method for the estimation of first derivative UV Visible Spectrophotometric method for Itraconazole was developed in its bulk and dosage form. Methanol was chosen as solvent system. The λmax was found to be 261 nm. The response was linear in the range of 20-60μg/ml. The regression equation of the calibration graph and correlation coefficient were found to be y=-0.001x-0.001 and 0.998 respectively. The sandell’s sensitivity (μg/cm3 at 0.001 absorbance unit) was 0.476. The, LOD (limit of detection), LOQ (limit of quantitation) and % RSD value for both intraday, interday precision was 0.5μg/ml, 1.65μg/ml and 0.97%,1.37% respectively. The proposed method is precise, rapid and accurate and can be used for the quantitation of Itraconazole for formulation.
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